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Robotic crystallisation screening
Crystallisation screening
Crystallisation screening at Bio21 C3 is when you use one of our standard screens to test your protein for crystallisability. At Bio21 C3 we offer a screening service where we set up your sample in one or more of the 96 condition crystallisation screens that you request.
As of mid-2008, the CSIRO node has 51 available screens in-house, including favourites like the CrystalScreen HT from Hampton Research, and the Wizard Full screen from Emerald BioSystems.
All of the screens are formatted into 96 deep well blocks, so a 24 well screen (such as The Grid Screen MPD, from Hampton Research) is combined with other screens to make up 96 conditions.
For more extensive screening, screen sets may be selected. These are combinations of 96-well screens that complement each other. Currently we offer two 8x96 well screens, the commercial set and the c3_screen. As well, we offer the popular 2x96 well NKI_set (which consists of JCSG+ and PACT screens run in tandem). This is the screen_set that the facility manager recommends as a starting point, in the absence of any other information.
Crystallisation experiments are set up in Innovadyne’s SD-2 sitting drop crystallisation plates, and are stored for two months at either 20C or 8C. By default, the crystallisation plates are inspected four times in the first week, (on setup, and on days 1, 3 and 5 after setup), twice in the second week (days 7 and 10) and once a week after that, for a total of 13 inspections. After the scheduled inspections are completed, the plates are held for collection at CSIRO.
We need about 180µl of protein solution to set up an 8x96 well screen, using our smallest droplets.
Click here to find a more complete description of how much protein solution we need from you.
Click here for more information on how to book a screening experiment through the booking interface.
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