Tissue culture provides protein for structural and biological studies

Tissue culture provides protein for structural and biological studies

Processing fermentation products

CSIRO’s fermentation group has expertise in molecular biology, protein chemistry and protein purification. The laboratories are also equipped for processing products of fermentation.

  • 2 September 2010 | Updated 14 October 2011

The capability

CSIRO has fermentation facilities for large-scale tissue culture and microbial work.

Our tissue culture laboratory can cultivate mammalian, hybridoma and insect cells while the microbial laboratory uses bacterial and yeast cells.

We produce recombinant proteins including complex glycoproteins, biomass and other cell products. We also develop large-scale fermentation methodologies.

The fermentation facilities are capable of fast characterisation, optimisation and scale-up of genetically engineered cells. 

In addition, the fermentation group offers expertise in computerised on-line fermenter control to ensure optimal yields and product quality.

CSIRO provides complex recombinant proteins, monoclonal antibodies, genetically engineered cell mass and microbial products to research institutions and industry.

Our facilities

The fermentation laboratories have:

  • cell culture bioreactors and microbial fermenters 
  • roller bottle apparatus, spinner flasks and versatile bioreactors for cell culture work 
  • batch and continuous perfusion techniques
  • microcarrier beads or packed-bed disk systems to carry out large-scale cell culturing of up to 250 litres of harvest 
  • host cells for tissue culture work including mammalian cell lines, hybridomas and insect cells (Sf9, Sf21, Hi5) 
  • production vessels with 10 litre working volumes for bacterial and yeast fermentation work using batch and fed-batch procedures. 
  • access to larger vessels, 100 litre and above, for scale-up work or production runs is possible.

How CSIRO uses it

We produce recombinant materials for medical research, diagnostic development and structural studies for commercial and research partners. These include:

  • cell surface receptors
  • monoclonal antibodies
  • signalling and carrier proteins
  • enzymes
  • antigens. 

Our integrated capabilities enable us to work with partners to:

  • engineer host cells
  • characterise target proteins
  • optimise target proteins
  • scale-up target proteins
  • produce target proteins
  • purify the proteins through:
    • separation
    • concentration
    • affinity chromatography
    • polishing.
  • move proteins into further studies.

Our partners

The Fermentation Facility is available to researchers and industry on a collaborative or contract basis. 

All negotiations and activities can be conducted under a confidentiality agreement if required. 

CSIRO can provide appropriate levels of intellectual property protection for work carried out in the facility.

We are proud to have worked with:

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