Innocuity testing

AAHL is NATA accredited to provide a wide range of testing to determine innocuity of materials, exclude adventitious agents and for other specialised purposes.

Innocuity testing is one of the many services we offer at AAHL.

The innnocuity team at the Australian Animal Health Laboratory (AAHL) offers a range of tests to detect or exclude the presence of adventitious agents in veterinary vaccines, including viruses, bacteria, fungi and mycoplasma.

Testing is generally done on seeds for veterinary vaccines, for example, seed cell lines, seed virus or bacteria on permissive cell lines over three to four passages followed by immunodetection or molecular testing.

The testing follows international standards such as AQIS, USDA 9CFR and European Pharmacopoeia and recently updated Department of Agriculture guidelines for testing imported veterinary vaccines.

Our innocuity testing capabilities

Innocuity testing
Pathogen Cell line Detection system
Acarina primary bovine thyroid cells and IB-RS-2 cells PCR
Rinderpest Vero monolayers IFAT and CpE
Caprine and ovine pox virus LT monolayers CpE; PCR
Swine vesicular disease virus IB-RS-2 monolayers CpE; PCR
African swine fever virus Porcine kidney monolayers CpE, PCR
Classical swine fever virus PK15-A CpE, IFAT
Bovine adenovirus Calf kidney monolayers CpE, IFAT
Akabane virus Aedes albopictus, BHK, CpE, IFAT
Bluetongue virus Eggs, Aedes, BHK-21 CpE, IFAT, PCR
Epizootic haemorrhagic disease virus BHK-21 CpE, IFAT, PCR
Bovine ephemeral fever virus Aedes albopictus, BHK CpE, IFAT
Bovine parvovirus BK monolayers CpE, IFAT
Bovine respiratory syncytial virus Bovine turbinate monolayers CpE, IFAT
Bovine viral diarrhoea LT monolayers, MDBK cells IFAT
Brucella abortus Biphasic medium Brucella colonies
Mycoplasma mycoides subsp. mycoides small colony (SC) type (CBPP) Mycoplasma broth Colonies, PCR
Coxiella burnetii (Q fever) Vero monolayers PCR (COM1 and IS1111)
BHV1 MDBK monolayers CpE, IFAT, PCR
BHV2 Primary calf testes (CTC) monolayers CpE, IFAT, PCR
(PCR not yet operational)
BHV4 MDBK monolayers CpE, IFAT, PCR
Bovine immodeficiency virus (BIV) PCR
Lumpy skin disease virus LT monolayers CpE, PCR
Enzootic bovine leukosis (EBL) PCR
Bovine parainfluenza virus 3 MDBK monolayers CpE, IFAT
Rabies Neuro 2A monolayers CpE, IFAT
Rift Valley fever virus Aedes albopictus monolayers monolayers CpE, IFAT
Rotavirus (bovine and porcine) MA-104 CpE, IFAT
Vesicular Stomatitis Virus Vero monolayers CpE, IFAT
African horse sickness virus Vero monolayers CpE
Taylorella equigenitalis TSB broth Colonies, PCR
Equine arteritis virus RK-13 monolayers CpE, IFAT
Equine encephalitis viruses (WEE, EEE VEE) Vero monolayers CpE, IFAT, PCR (group specific)
Equid herpesvirus 1,4 Primary equine foetal kidney monolayers CpE, IFAT, PCR
Equine influenza virus Chicken embryos, MDCK CpE, embryo death, PCR
Burkholderia mallei Inoculate TSA PCR
Trypanosoma evansi (Surra) PCR
Brucella melitensis Biphasic medium Brucella colonies
Caprine arthritis encephalitis virus and Visna/Maedi virus PCR
Mycoplasma agalactictiae Mycoplasma broth PCR
Mycoplasma capricolum subsp. capripneumoniae (Contagious caprine pleuro-pneumonia) Mycoplasma broth PCR
Orf virus LT monolayers CpE, IFAT
Ovine adenovirus LT monolayer IFAT
Border disease in sheep LT monolayers IFAT
Porcine adenovirus Primary pig kidney monolayers CpE, IFAT
Pseudorabies virus (Aujeszky’s disease) PK-15A monolayers CpE, IFAT, PCR
Brucella suis See Brucella abortus

See Brucella abortus

Porcine haemagglutinating encephalomyelitis virus          Primary pig kidney monolayers


Mycoplasma hyopneumoniae MS broth PCR
Porcine Teschovirus PTV-1, former PEV-1 Primary pig kidney monolayers CpE, IFAT
Porcine epidemic diarrhoea virus                Vero monolayers CpE, PCR
Porcine parvovirus    Primary PK cell CpE, IFAT
Porcine enteroviruses   CpE
Porcine respiratory coronavirus   PCR
Porcine reproductive and respiratory syndrome virus  


Porcine circovirus 1, 2 PK15-A cell IFAT, PCR
Swine influenza virus Egg, MDCK monolayers CpE, egg death, PCR
Swine pox virus Primary pig kidney monolayers


Transmissible gastroenteritis virus (TGE) and porcine respiratory corona virus (PRCV)

Primary pig kidney monolayers


Avian influenza virus Chicken embryos Egg deaths, HA, PCR
Avian adenoviruses CEF monolayers CpE, ELISA (Ag)
Newcastle disease virus Chicken embryos Egg deaths, HA, PCR
Infectious bronchitis virus Chicken embryos Egg deaths, PCR
Infectious bursal disease virus Chicken embryos Egg deaths, PCR
Ornithobacterium rhinotracheale   PCR
Salmonella enteritidis, gallinarum and pullorum TSB and selenite broth Colonies, PCR
Turkey rhinotracheitis virus Chicken embryos, Vero monolayers

Egg deaths, CpE, PCR

Brucella canis Biphasic medium Colonies
Leptospira canicola Culture  
Canine adenovirus 1 and 2 MDCK monolayers CpE, IFAT
Canine distemper virus MDCK monolayers


Canine parvovirus CRFK monolayers CpE, IFAT
Ehrlichia canis PCR
Feline calicivirus CRFK cells CpE, IFAT
Feline infectious peritonitis virus CRFK monolayers CpE, IFAT
Feline panleukopaenia virus CRFK monolayers CpE, IFAT
Felid herpesvirus 1 (Feline rhinotracheitis) CRFK monolayers CpE, IFAT
Japanese Encephalitis and West Nile Vero monolayers CpE, PCR
General testing
Pathogen Cell line

Cytopathic and haemadsorbing agents

AAHL holds a wide range of primary and continuous cell lines from different species, e.g. bovine, ovine, porcine, equine, caprine, canine and feline. General testing for avian pathogens is done on CEK cells.

Use this service

  1. Review our innocuity testing capabilities
  2. Contact our innocuity technical manager

Enquiries about non-listed test capabilities are also welcome.

AAHL innocuity technical manager

  • Phone:
    • + 61 3 5227 5414 (AAHL accessions)


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